High-level expression of functional cGMP-dependent protein kinase using the baculovirus system
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چکیده
منابع مشابه
High level expression of mammalian protein farnesyltransferase in a baculovirus system. The purified protein contains zinc.
The mammalian enzyme protein farnesyltransferase is a heterodimeric protein that catalyzes the addition of a farnesyl isoprenoid to a cysteine in ras proteins. Since oncogenic forms of ras proteins require the farnesyl group for transforming activity, the structure and mechanism of this enzyme are important to define. However, such studies have been difficult to approach because of the low abun...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1993
ISSN: 0014-5793
DOI: 10.1016/0014-5793(93)81632-a